Structural Characterization and Immunomodulatory Activity of a Novel Polysaccharide from Lepidium meyenii

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• 作者：Mengmeng Zhang ; Guang Wang ; Furao Lai ; Hui Wu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：March 9, 2016
• 年：2016
• 卷：64
• 期：9
• 页码：1921-1931
• 全文大小：632K
• ISSN：1520-5118

文摘

A novel polysaccharide named as MC-1 was isolated from the roots of Lepidium meyenii using a water extraction method. Structural characterization revealed that MC-1 had an average molecular weight of 11.3 kDa and consisted of arabinose (26.21%), mannose (11.81%), glucose (53.66%), and galactose (8.32%). The main linkage types of MC-1 were proven to be (1 → 5)-α-l-Ara, (1 → 3)-α-l-Man, (1 → 2,6)-α-l-Man, (1 → )-α-d-Glc, (1 → 4)-α-d-Glc, (1 → 6)-α-d-Glc and (1 → 6)-β-d-Gal by methylation analysis, periodate oxidation-Smith degradation and NMR analysis. The immunostimulating assay indicated that MC-1 could significantly enhance the pinocytic and phagocytic capacity and promote the NO, TNF-α, and IL-6 secretion of RAW 264.7 cells, involving toll-like receptor 2, complement receptor 3, and mannose receptor mainly. These results suggested the potential utilization of MC-1 as an attractive functional food supplement candidate for hypoimmunity population.