Malate Metabolism and Adaptation to Chilling Temperature Storage by Pretreatment with High CO2 Levels in Annona cherimola Fruit

详细信息    查看全文

• 作者：Roberto Maldonado ; M. Teresa Sanchez-Ballesta ; Rafael Alique ; M. Isabel Escribano ; and Carmen Merodio
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2004
• 出版时间：July 28, 2004
• 年：2004
• 卷：52
• 期：15
• 页码：4758 - 4763
• 全文大小：77K
• 年卷期：v.52,no.15(July 28, 2004)
• ISSN：1520-5118

文摘

In this study we focused on the effect of a pretreatment with high (20%) CO₂ levels on malic acidmetabolism in cherimoya (Annona cherimola Mill) fruit stored at chilling temperature. We analyzedthe activity of phosphoenolpyruvate carboxylase (PEPC), malate dehydrogenase (MDH), and theNADP-malic enzyme (NADP-ME), involved in the carboxylation/decarboxylation of malate. Ourresults show that CO₂ treatment, which improves tolerance to prolonged storage at chilling temperature,was closely linked to considerably greater NADP-ME activity. These results, combined with lowerPEPC activity, may explain the significantly lower amount of malic acid and titratable acidity quantifiedin CO₂-treated fruit. Moreover, the high cytoplasmic MDH enzyme activity and the strong stimulationof NADP-ME activity exhibited by CO₂-treated fruit could be contributing factors in the maintenanceof fruit energy metabolism, pH stability, and the promotion of synthesis of defense compounds thatprevent or repair damage caused by chilling temperature.Keywords: Cherimoya; chilling temperature; titratable acidity; malate; carbon dioxide; phosphoenolpyruvate carboxylase; malate dehydrogenase; malic enzyme