文摘
In the study, a novel chitosan (CS) derivative conjugated with multiple galactose residues in an antennary fashion(Gal-m-CS) was synthesized. A galactosylated CS (Gal-CS) was also prepared by directly coupling lactobionicacid on CS. Using an iontropic gelation method, CS and the synthesized Gal-CS and Gal-m-CS were used toprepare nanoparticles (CS, Gal-CS, and Gal-m-CS NPs) for targeting hepatoma cells. TEM examinations showedthat the morphology of all three types of NPs was spherical in shape. No aggregation or precipitation of NPs inan aqueous environment was observed during storage for all studied groups, as a result of the electrostatic repulsionbetween the positively charged NPs. Little fluorescence was observed in HepG2 cells after incubation with theFITC-labeled CS NPs. The intensity of fluorescence observed in HepG2 cells incubated with the Gal-m-CS NPswas stronger than that incubated with the Gal-CS NPs. These results indicated that the prepared Gal-m-CS NPshad the highest specific interaction with HepG2 cells among all studied groups, via the ligand-receptor-mediatedrecognition.