Preparation and Characterization of Nanoparticles Shelled with Chitosan for Oral Insulin Delivery

详细信息    查看全文

• 作者：Yu-Hsin Lin ; Fwu-Long Mi ; Chiung-Tong Chen ; Wei-Chun Chang ; Shu-Fen Peng ; Hsiang-Fa Liang ; Hsing-Wen Sung
• 刊名：Biomacromolecules
• 出版年：2007
• 出版时间：January 2007
• 年：2007
• 卷：8
• 期：1
• 页码：146 - 152
• 全文大小：566K
• 年卷期：v.8,no.1(January 2007)
• ISSN：1526-4602

文摘

Nanoparticles (NPs) composed of chitosan (CS) and poly(-glutamic acid) (-PGA) were prepared by a simpleionic-gelation method for oral insulin delivery. Fourier transform infrared (FT-IR) spectra indicated that CS and-PGA were ionized at pH 2.5-6.6, while X-ray diffractograms demonstrated that the crystal structure of CSwas disrupted after it was combined with -PGA. The diameters of the prepared NPs were in the range of 110-150 nm with a negative or positive surface charge, depending on the relative concentrations of CS to -PGAused. The NPs with a positive surface charge (or shelled with CS) could transiently open the tight junctionsbetween Caco-2 cells and thus increased the paracellular permeability. After loading of insulin, the NPs remainedspherical and the insulin release profiles were significantly affected by their stability in distinct pH environments.The in vivo results clearly indicated that the insulin-loaded NPs could effectively reduce the blood glucose levelin a diabetic rat model.