Proteogenomic Analysis of Candida glabrata using High Resolution Mass Spectrometry
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- 作者：T. S. Keshava Prasad ; H. C. Harsha ; Shivakumar Keerthikumar ; Nirujogi Raja Sekhar ; Lakshmi Dhevi N. Selvan ; Praveen Kumar ; Sneha M. Pinto ; Babylakshmi Muthusamy ; Yashwanth Subbannayya ; Santosh Renuse ; Raghothama Chaerkady ; Premendu P. Mathur ; Raju Ravikumar ; Akhilesh Pandey
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：January 1, 2012
- 年：2012
- 卷：11
- 期：1
- 页码：247-260
- 全文大小：742K
- 年卷期：v.11,no.1(January 1, 2012)
- ISSN：1535-3907
文摘
Candida glabrata is a common opportunistic human pathogen leading to significant mortality in immunosuppressed and immunodeficient individuals. We carried out proteomic analysis of C. glabrata using high resolution Fourier transform mass spectrometry with MS resolution of 60000 and MS/MS resolution of 7500. On the basis of 32453 unique peptides identified from 118815 peptide鈥搒pectrum matches, we validated 4421 of the 5283 predicted protein-coding genes (83%) in the C. glabrata genome. Further, searching the tandem mass spectra against a six frame translated genome database of C. glabrata resulted in identification of 11 novel protein coding genes and correction of gene boundaries for 14 predicted gene models. A subset of novel protein-coding genes and corrected gene models were validated at the transcript level by RT-PCR and sequencing. Our study illustrates how proteogenomic analysis enabled by high resolution mass spectrometry can enrich genome annotation and should be an integral part of ongoing genome sequencing and annotation efforts.