Metabolic Pathway of Cyanidin 3-O--D-Glucopyranoside in Rats
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- 作者：Takashi Ichiyanagi ; Yasuo Shida ; M. Mamunur Rahman ; Yoshihiko Hatano ; Hitoshi Matsumoto ; Masao Hirayama ; and Tetsuya Konishi
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：January 12, 2005
- 年：2005
- 卷：53
- 期：1
- 页码：145 - 150
- 全文大小：171K
- 年卷期：v.53,no.1(January 12, 2005)
- ISSN：1520-5118
文摘
For better understanding of the physiological function of anthocyanins, the absorption and metabolismof cyanidin 3-O-
-D-glucopyranoside (Cy3G), which is one of the major anthocyanins in colored foodmaterials, were precisely investigated. Combining two modalities newly developed, that is, highlysensitive semi-micro-HPLC and vein cannulation, Cy3G and its four major metabolites (M1-M4)were detected in the blood plasma of rats after oral administration of Cy3G (100 mg/kg of body mass).The plasma concentration of Cy3G reached its maximum at 15 min after the ingestion. Metabolite 2(M2) and metabolite 3 (M3) showed their maximum plasma levels at 15 and 30 min, respectively,whereas metabolite 1 (M1) and metabolite 4 (M4) showed their maximum levels at 60 and 120 min,respectively. The maximum plasma concentrations of the four metabolites were in the followingorder: M3 (21 nM) > M4 (20 nM) > M1 (8.5 nM) > M2 (5 nM). When Cy3G was directly injected intothe neck vein, only M2 and M3 were detected in the plasma, indicating that both M1 and M4 wereproduced during absorption from the gastrointestinal tract. Tandem MS analysis of the metabolitesshowed that M2 and M3 were monomethylated Cy3G, while M1 and M4 were glucuronides of Cyand methylated Cy, respectively. M3 was assigned as peonidin 3-O--D-glucopyranoside (Pn3G)from the comparison of the retention time of authentic Pn3G.Keywords: Cyanidin 3-O--D-glucopyranoside; peonidin 3-O--D-glucopyranoside; 4'-O-methylcyanidin3-O--D-glucopyranoside; absorption; metabolic pathway; glucuronide
-D-glucopyranoside (Cy3G), which is one of the major anthocyanins in colored foodmaterials, were precisely investigated. Combining two modalities newly developed, that is, highlysensitive semi-micro-HPLC and vein cannulation, Cy3G and its four major metabolites (M1-M4)were detected in the blood plasma of rats after oral administration of Cy3G (100 mg/kg of body mass).The plasma concentration of Cy3G reached its maximum at 15 min after the ingestion. Metabolite 2(M2) and metabolite 3 (M3) showed their maximum plasma levels at 15 and 30 min, respectively,whereas metabolite 1 (M1) and metabolite 4 (M4) showed their maximum levels at 60 and 120 min,respectively. The maximum plasma concentrations of the four metabolites were in the followingorder: M3 (21 nM) > M4 (20 nM) > M1 (8.5 nM) > M2 (5 nM). When Cy3G was directly injected intothe neck vein, only M2 and M3 were detected in the plasma, indicating that both M1 and M4 wereproduced during absorption from the gastrointestinal tract. Tandem MS analysis of the metabolitesshowed that M2 and M3 were monomethylated Cy3G, while M1 and M4 were glucuronides of Cyand methylated Cy, respectively. M3 was assigned as peonidin 3-O--D-glucopyranoside (Pn3G)from the comparison of the retention time of authentic Pn3G.Keywords: Cyanidin 3-O--D-glucopyranoside; peonidin 3-O--D-glucopyranoside; 4'-O-methylcyanidin3-O--D-glucopyranoside; absorption; metabolic pathway; glucuronide