文摘
Proanthocyanidin oligomers with different degrees of polymerization were isolated from Saskatoonberries (Amelanchier alnifolia) by means of gel adsorption and normal-phase liquid chromatography.The proanthocyanidins were identified using electrospray ionization mass spectrometry, nuclearmagnetic resonance spectroscopy, and thiolytic degradation coupled with reversed-phase liquidchromatography. The results established that Saskatoon berries contain proanthocyanidins fromdimers through heptamers and higher polymers. Saskatoon proanthocyanidins are essentially ofprocyanidin type, consisting mainly of epicatechin units linked by B-type bonds. The simple procyanidinprofile of Saskatoon berries allowed the procyanidins to be separated precisely according to theirdegrees of polymerization. In the future they can be used as standard compounds for qualitative andquantitative analysis of procyanidins as well as for elucidation of the biological activities ofproanthocyanidins.