Identification of the Grincamycin Gene Cluster Unveils Divergent Roles for GcnQ in Different Hosts, Tailoring the l-Rhodinose Moiety

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• 作者：Yun Zhang ; Hongbo Huang ; Qi Chen ; Minghe Luo ; Aijun Sun ; Yongxiang Song ; Junying Ma ; Jianhua Ju
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：July 5, 2013
• 年：2013
• 卷：15
• 期：13
• 页码：3254-3257
• 全文大小：234K
• 年卷期：v.15,no.13(July 5, 2013)
• ISSN：1523-7052

文摘

The gene cluster responsible for grincamycin (GCN, 1) biosynthesis in Streptomyces lusitanus SCSIO LR32 was identified; heterologous expression of the GCN cluster in S. coelicolor M512 yielded P-1894B (1b) as a predominant product. The 螖gcnQ mutant accumulates intermediate 1a and two shunt products 2a and 3a bearing mallcaps">l-rhodinose for mallcaps">l-cinerulose A substitutions. In vitro data demonstrated that GcnQ is capable of iteratively tailoring the two mallcaps">l-rhodinose moieties into mallcaps">l-aculose moieties, supporting divergent roles of GcnQ in different hosts.