Application of N-Halogeno-N-sodiobenzenesulfonamide Reagents to the Selective Detection of 5-Methylcytosine in DNA Sequences

详细信息    查看全文

• 作者：Tianlu Wang ; Tingting Hong ; Tun Tang ; Qianqian Zhai ; Xiwen Xing ; Wuxiang Mao ; Xiaolong Zheng ; Liang Xu ; Jinjun Wu ; Xiaocheng Weng ; Shaoru Wang ; Tian Tian ; Bifeng Yuan ; Bing Huang ; Lin Zhuang ; Xiang Zhou
• 刊名：The Journal of the American Chemical Society
• 出版年：2013
• 出版时间：January 30, 2013
• 年：2013
• 卷：135
• 期：4
• 页码：1240-1243
• 全文大小：240K
• 年卷期：v.135,no.4(January 30, 2013)
• ISSN：1520-5126

文摘

To surmount the challenges of the locus determination and accurate quantification of 5-methyl-2鈥?deoxycytidine (^5MedC) in DNA fragments that contain multiple ^5MedC residues, we designed and synthesized two N-halogeno-N-sodiobenzenesulfonamide reagents that provide a new chemical method for probing ^5MedC in DNA sequences. When the strategy we provided was combined with 尾-glucosyltransferase, ^5MedC could be distinguished from 5-hydroxymethyl-2鈥?deoxycytidine (^5hmdC) and deoxycytidine (dC) through the introduction of a glucose moiety to the hydroxyl group of ^5hmdC.