Effect of Flavonoids on Stress Responses in Myotube Cultures

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• 作者：Jette F. Young ; Jens Hansen-M//pubs.acs.org/images/gifchars/oslash.gif" border="0">ller ; and Niels Oksbjerg
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2004
• 出版时间：November 17, 2004
• 年：2004
• 卷：52
• 期：23
• 页码：7158 - 7163
• 全文大小：92K
• 年卷期：v.52,no.23(November 17, 2004)
• ISSN：1520-5118

文摘

Effects of flavonoids on stress response of myotube cultures was studied by monitoring the releaseof [¹⁴C] taurine, leukotriene production, and 2',7'-dichlorodihydroflourescein (DCFH₂) oxidation. Stresswas induced by hypotonic shock, which was accompanied by cell swelling leading to increasedleukotriene production and a concomitant increase in reactive oxygen species and osmolyte release.In this model system, addition of the flavonoids catechin and quercetin decreased leukotrieneproduction, DCFH₂ oxidation, and taurine efflux, indicating a reduction of cellular stress. Highconcentrations of epigallocatechin gallate (EGCG) and tea extract increased leukotriene productionand initial DCFH₂ oxidation, indicating an increased cellular stress (possibly toxicity). However, taurineefflux was reduced, and also longer exposure time as well as lower concentrations of EGCG and teareduced DCFH₂ oxidation. Trolox and fchars/alpha.gif" BORDER=0>-tocopherol did not significantly affect taurine efflux orleukotriene production, and it was therefore concluded that suppression of these responses was notconfined to redox activity in a myotube culture.Keywords: Cell culture; myotubes; stress; flavonoids; tea; leukotriene; taurin efflux; DCFH₂ oxidation