Rapid Screening of Polar Compounds in Brazilian Propolis by High-Temperature High-Resolution Gas Chromatography-Mass Spectrometry

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• 作者：A. S. Pereira ; M. Norsell ; J. N. Cardoso ; F. R. Aquino Neto ; and M. F. S. Ramos
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2000
• 出版时间：November 2000
• 年：2000
• 卷：48
• 期：11
• 页码：5226 - 5230
• 全文大小：73K
• 年卷期：v.48,no.11(November 2000)
• ISSN：1520-5118

文摘

Methanol extracts of propolis from six different places, five in Rio de Janeiro state and one in SãoPaulo state, both in the Southeast of Brazil, were investigated using high-temperature high-resolution gas chromatography (HT-HRGC) and HT-HRGC-mass spectometry. The main purposeof the study was to establish the applicability of HT-HRGC as an analytical method for systematicstudies of polar propolis fractions. Several compounds, including carbohydrates, phenolic acidderivatives, and high molecular weight compounds (e.g., wax esters of long chain fatty alcohols)could be readily characterized in the crude extracts by HT-HRGC-MS. HT-HRGC and HT-HRGC-MS were shown to be quick and informative tools for rapid analysis of crude polar extracts withoutcleanup.Keywords: Propolis; high-temperature gas chromatography; polar compounds; carbohydrates; highmolecular weight compounds