Anti-inflammatory Activities of New Succinic and Maleic Derivatives from the Fruiting Body of Antrodia camphorata

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• 作者：Shih-Chang Chien ; Miaw-Ling Chen ; Hsiou-Ting Kuo ; Yao-Ching Tsai ; Bi-Fong Lin ; Yueh-Hsiung Kuo
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：August 27, 2008
• 年：2008
• 卷：56
• 期：16
• 页码：7017-7022
• 全文大小：177K
• 年卷期：v.56,no.16(August 27, 2008)
• ISSN：1520-5118

文摘

Six new compounds, trans-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione (1), trans-1-hydroxy-3-(4-hydroxyphenyl)-4-isobutylpyrrolidine-2,5-dione (2), cis-3-(4-hydroxyphenyl)-4-isobutyldihydrofuran-2,5-dione (3), 3-(4-hydroxyphenyl)-4-isobutyl-1H-pyrrole-2,5-dione (4), 3-(4-hydroxyphenyl)-4-isobutylfuran-2,5-dione (5), and dimethyl 2-(4-hydroxyphenyl)-3-isobutylmaleate (6), together with one known compound, 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]furan-2,5-dione (7), were isolated from the fruiting bodies of Antrodia camphorata. The structures of the compounds were elucidated by analysis of their spectroscopic data. To investigate the immunomodulatory potential of the compounds, RAW264.7 macrophage cells were treated with the compounds. Compound 1 significantly increased spontaneous TNF-α secretion from unstimulated RAW264.7 cells but suppressed IL-6 production [50% inhibition concentration value (IC₅₀) = 10 μg/mL] in LPS-stimulated cells. Compounds 3, 4, and 6 also suppressed IL-6 production with IC₅₀ values of 17, 18, and 25 μg/mL, respectively, suggesting that these four compounds may have an anti-inflammatory effect on macrophage-mediated responses. Of the six compounds, compound 1 was the most effective, exerting both immunostimulatory and anti-inflammatory effects.