This
pa
per summarizes the recent activities of the Chromosome-Centric Human Proteome Project (C-HPP) consortium, which develo
ps new technologies to identify yet-to-be annotated
proteins (
termed 鈥渕issing
proteins鈥? in biological sam
ples that lack sufficient ex
perimental evidence at the
protein level for confident
protein identification. The C-HPP also aims to identify new
protein forms that may be caused by genetic variability,
post-translational modifications, and al
ternative s
plicing. Proteogenomic data integration forms the basis of the C-HPP鈥檚 activities; therefore, we have summarized some of the key a
pproaches and their roles in the
project. We
present new analytical technologies that im
prove the chemical s
pace and lower detection limits cou
pled to bioinformatics tools and some
publicly available resources that can be used to im
prove data analysis or su
pport the develo
pment of analytical assays. Most of this
pa
per鈥檚 content has been com
piled from
pos
ters, slides, and discussions
presented in the series of C-HPP worksho
ps held during 2014. All data (
pos
ters,
presentations) used are available at the C-HPP Wiki (
p://c-hpp.webhosting.rug.nl/" class="extLink">http://c-hpp.webhosting.rug.nl/) and in the Supporting Information.