Parallel Multifunctionalization of Nanoparticles: A One-Step Modular Approach for in Vivo Imaging

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• 作者：Hugo Groult ; Jes煤s Ruiz-Cabello ; Juan Pellico ; Ana V. Lechuga-Vieco ; Riju Bhavesh ; Moreno Zamai ; Elena Almarza ; In茅s Mart铆n-Padura ; Eugenio Cantelar ; Mar铆a P. Mart铆nez-Alc谩zar ; Fernando Herranz
• 刊名：Bioconjugate Chemistry
• 出版年：2015
• 出版时间：January 21, 2015
• 年：2015
• 卷：26
• 期：1
• 页码：153-160
• 全文大小：394K
• ISSN：1520-4812

文摘

Multifunctional nanoparticles are usually produced by sequential synthesis, with long multistep protocols. Our study reports a generic modular strategy for the parallel one-step multifunctionalization of different hydrophobic nanoparticles. The method was designed and developed by taking advantage of the natural noncovalent interactions between the fatty acid binding sites of the bovine serum albumin (BSA) and the aliphatic surfactants on different inorganic nanomaterials. As a general example of the approach, three different nanoparticles鈥攊ron oxide, upconverting nanophosphors, and gold nanospheres鈥攚ere nanoemulsified in water with BSA. To support specific applications, multifunctional capability was incorporated with a variety of previously modified BSA modules. These modules include different conjugated groups, such as chelating agents for ⁶⁸Ga or ⁸⁹Zr and ligand molecules for enhanced in vivo targeting. A large library of 13 multimodal contrast agents was developed with this convergent strategy. This platform allows a highly versatile and easy tailoring option for efficient incorporation of functional groups. Finally, as demonstration of this versatility, a bimodal (PET/MRI) probe including a maleimide-conjugated BSA was selectively synthesized with an RGD peptide for in vivo imaging detection of tumor angiogenesis.