Mapping Mouse Gamete Interaction Forces Reveal Several Oocyte Membrane Regions with Different Mechanical and Adhesive Properties

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• 作者：Antoine J&eacute ; gou ; Fr&eacute ; d&eacute ; ric Pincet ; Eric Perez ; Jean Philippe Wolf ; Ahmed Ziyyat ; Christine Gourier
• 刊名：Langmuir
• 出版年：2008
• 出版时间：February 19, 2008
• 年：2008
• 卷：24
• 期：4
• 页码：1451 - 1458
• 全文大小：372K
• 年卷期：v.24,no.4(February 19, 2008)
• ISSN：1520-5827

文摘

This study focuses on the interaction involved in the adhesion of mouse gametes and on the mechanical propertiesof the oocyte membrane. The oocyte has an asymmetrical shape, and its membrane is composed of two distinct areas.One is rich in microvilli, and the other is smoother and without microvilli. With a biomembrane force probe (BFP)adapted to cell-cell measurements, we have quantified the separation forces between a spermatozoon and an oocyte.Microvillar and amicrovillar areas of the oocyte surface have been systematically probed and compared. In additionto a substantial difference in the elastic stiffness of these two regions, the experiments have revealed the presenceof two types of membrane domains with different mechanical and adhesive properties, both distributed over the entireoocyte surface (i.e., in both microvillar and amicrovillar regions). If gamete contact occurs in the first type of domain,then the oocyte membrane deforms only elastically under traction. The pull-off forces in these domains are higherin the amicrovillar region. For a spermatozoon contact with the other type of domain, there can be a transition fromthe elastic to viscoelastic regime, and then tethers are extruded from the oocyte membrane.