Identification and Design of Peptides for the Rapid, High-Yield Formation of Nanoparticulate TiO2 from Aqueous Solutions at Room Temperature

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• 作者：Matthew B. Dickerson ; Sharon E. Jones ; Ye Cai ; Gul Ahmad ; Rajesh R. Naik ; Nils Krö ; ger ; Kenneth H. Sandhage
• 刊名：Chemistry of Materials
• 出版年：2008
• 出版时间：February 26, 2008
• 年：2008
• 卷：20
• 期：4
• 页码：1578 - 1584
• 全文大小：270K
• 年卷期：v.20,no.4(February 26, 2008)
• ISSN：1520-5002

文摘

Titania (TiOB>2) nanoparticles are widely used, or are under active development, for a range of applications in (photo)catalysis, photovoltaics, enzyme support, energy storage, and photonics. The peptide-directed room-temperature formation of titania nanoparticles can be an attractive alternative to higher-temperature synthetic methods. However, the influence of the peptide primary structure on the titania precipitation activity at room temperature is not well understood. Through the selective binding of phage-displayed 12-mer peptides to TiO₂ substrates, we have identified 20 peptides with an affinity for titania. The average numbers of arginine, lysine, and histidine residues present in these 20 peptides were distinctly higher than for the overall peptide-bearing phage library. Synthetic 16-mer versions of four of these peptides (i.e., 12-mer peptides with C-terminal tetrapeptide tags for quantitative spectrophotometry) induced the formation of 8.1–38.7 mol TiO₂/mol peptide after exposure for only 10 min to an otherwise water-stable Ti(IV) complex at room temperature and a pH of 6.3. X-ray diffraction analyses, electron diffraction analyses, and high-resolution transmission electron microscopy revealed that the peptide-induced titania contained fine (<10 nm) anatase and monoclinic b2;-TiO₂ nanocrystals, along with an amorphous phase. The titania yield increased with the number of positive charges carried by these peptides. On the basis of these results, a peptide was designed that exhibited the highest titania formation activity reported to date for a peptide (82.9 mol TiO₂/mol peptide), as well as a reduced pH dependence for such titania formation.