Degradability of Polysaccharides Multilayer Films in the Oral Environment: an in Vitro and in Vivo Study

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• 作者：Olivier Etienne ; Aurore Schneider ; Corinne Taddei ; Ludovic Richert ; Pierre Schaaf ; Jean-Claude Voegel ; Christophe Egles ; and Catherine Picart
• 刊名：Biomacromolecules
• 出版年：2005
• 出版时间：March 2005
• 年：2005
• 卷：6
• 期：2
• 页码：726 - 733
• 全文大小：627K
• 年卷期：v.6,no.2(March 2005)
• ISSN：1526-4602

文摘

Biomedical devices and modified biomaterial surfaces constitute an expanding research domain in the dentalfield. However, such oral applications have to face a very particular environment containing specificphysiological conditions and specific enzymes. To evaluate their suitability in the development of noveloral applications, the degradability of polyelectrolyte multilayer films made of the natural polysaccharideschitosan and hyaluronan (CHI/HA) was investigated in vitro and in vivo in a rat mouth model. The filmswere either native or cross-linked using a water-soluble carbodiimide (EDC) in combination withN-hydroxysulfosuccinimide. The in vitro degradation of the films by different enzymes present in the oralenvironment, such as lysozyme and amylase, was followed by quartz crystal microbalance measurementsand confocal laser scanning microscopy observations after being film labeled with CHI^FITC. Whereas nativefilms were subjected to degradation by all the enzymes, cross-linked films were more resistant to enzymaticdegradation. Films were also put in contact with whole saliva, which induced a slow degradation of thenative films over an 18 h period. The in vivo degradation of the films deposited on polymer disks andsutured in the rat mouth was followed over a 3 day period. Whereas film degradation is fast for nativefilms, it is much slower for the cross-linked ones. More than 60% of these films remained on the disks after3 days in the mouth. Taken together, these results suggest that the multilayer films made of naturalpolysaccharides are of high potential interest for oral applications, especially as drug release systems, offeringvarious degradation rates and consequent release characteristics.