Extraction and Antibacterial Properties of Thyme Leaf Extracts: Authentic Practice of Green Chemistry
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- 作者：Sean C. Purcell ; Prithvi Pande ; Yingxin Lin ; Ernesto J. Rivera ; Latisha Paw U ; Luisa M. Smallwood ; Geri A. Kerstiens ; Laura B. Armstrong ; MaryAnn T. Robak ; Anne M. Baranger ; Michelle C. Douskey
- 刊名：Journal of Chemical Education
- 出版年：2016
- 出版时间：August 9, 2016
- 年：2016
- 卷：93
- 期：8
- 页码：1422-1427
- 全文大小：266K
- 年卷期：0
- ISSN：1938-1328
文摘
In this undergraduate analytical chemistry experiment, students quantitatively assess the antibacterial activity of essential oils found in thyme leaves (Thymus vulgaris) in an authentic, research-like environment. This multiweek experiment aims to instill green chemistry principles as intrinsic to chemical problem solving. Students progress through various techniques including extraction, chromatography (TLC and HPLC), culturing bacteria, and disk diffusion via a process of guided exploration that emphasizes green experimental design. Approximately 600 undergraduate students carried out the experiment and self-reported substantial learning gains.