Application of Solid Phase Microextraction for Quantitation of Polyunsaturated Fatty Acids in Biological Fluids
详细信息 查看全文
- 作者：Afsoon Pajand Birjandi ; Fatemeh Sadat Mirnaghi ; Barbara Bojko ; Marcin W膮sowicz ; Janusz Pawliszyn
- 刊名：Analytical Chemistry
- 出版年：2014
- 出版时间：December 16, 2014
- 年：2014
- 卷：86
- 期：24
- 页码：12022-12029
- 全文大小：364K
- ISSN：1520-6882
文摘
Development of a straightforward strategy for simultaneous quantitative analysis of nonesterified fatty acids (NEFA) species in biofluids is a challenging task because of the extreme complexity of fatty acid distribution in biological matrices. In this study, we present a direct immersion solid phase microextraction method coupled to a liquid chromatography鈥搈ass spectrometry platform (DI-SPME- HPLC-ESI -MS) for determination of unconjugated fatty acids (FA) in fish and human plasma. The proposed method was fully validated according to bioanalytical method validation guidelines. The LOD and LOQ were in the range of 0.5鈥? and 5鈥?2 ng/mL, respectively, with a linear dynamic range of 100 fold for each compound. Absolute and relative matrix effects were comprehensively evaluated and found to be in the acceptable range of 91鈥?16%. The affinity constant (Ka) of individual FAs to protein albumin was determined to be 9.2 脳 104 to 4.3 脳 105 M鈥?. The plasma protein binding (PPB%) was calculated and found to be in the range of 98.0鈥?9.7% for different polyunsaturated fatty acids (PUFAs). The PUFAs under study were found at a high concentration range in fish plasma, whereas only a few were within quantification range in control human plasma. The method was successfully applied for monitoring PUFA changes during the operation in plasma samples obtained from patients undergoing cardiac surgery with the use of cardiopulmonary bypass (CPB). The most significant contribution induced by surgery was noticed in the concentration level of 伪-linolenic acid (18:3, ALA), arachidonic acid (20:4, AA), and docosahexanoic acid (22:6, DHA) soon after administration of CPB in all cases.