Composition and Distribution of Carrabiose Moieties in Hybrid κ-/ι-Carrageenans Using Carrageenases

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• 作者：Marion Guibet ; Patrick Boulenguer ; Jacques Mazoyer ; Nelly Kervarec ; Aristotelis Antonopoulos ; Michel Lafosse ; William Helbert
• 刊名：Biomacromolecules
• 出版年：2008
• 出版时间：January 2008
• 年：2008
• 卷：9
• 期：1
• 页码：408 - 415
• 全文大小：211K
• 年卷期：v.9,no.1(January 2008)
• ISSN：1526-4602

文摘

Hybrid κ/ι-carrageeans extracted from Gigartina skottsbergii, Chondracanthus chamissoï, and Chondrus crispus were incubated with Pseudoalteromonas carrageenovora κ-carrageenase and Alteromonas fortis ι-carrageenase. The degradation products as well as the resistant fraction were fully characterized by chromatography, NMR, and mass spectrometry. The low percentage of degradation observed after treatment by the ι-carrageenase suggests that long segments of ι-carrabiose or block of ι-carrageenan are low in abundance. The degradation products of the κ-carrageenase allow discriminating three modes of κ-carrabiose distribution: blocks of κ-carrabiose, κ-carrabiose rich fraction containing ι-carrabiose units probably randomly distributed, and ι-carrabiose rich fraction which corresponds to the resistant fraction. The proportions of each fraction were related to the botanical origin as well as the place of growth of the seaweed.