Impact of Drug Conjugation on Pharmacokinetics and Tissue Distribution of Anti-STEAP1 Antibody鈥揇rug Conjugates in Rats

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• 作者：C. Andrew Boswell ; Eduardo E. Mundo ; Crystal Zhang ; Daniela Bumbaca ; Nicole R. Valle ; Katherine R. Kozak ; Aimee Fourie ; Josefa Chuh ; Neelima Koppada ; Ola Saad ; Herman Gill ; Ben-Quan Shen ; Bonnee Rubinfeld ; Jay Tibbitts ; Surinder Kaur ; Frank-Peter Theil ; Paul J. Fielder ; Leslie A. Khawli ; Kedan Lin
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：October 19, 2011
• 年：2011
• 卷：22
• 期：10
• 页码：1994-2004
• 全文大小：1009K
• 年卷期：v.22,no.10(October 19, 2011)
• ISSN：1520-4812

文摘

Antibody鈥揹rug conjugates (ADCs) are designed to combine the exquisite specificity of antibodies to target tumor antigens with the cytotoxic potency of chemotherapeutic drugs. In addition to the general chemical stability of the linker, a thorough understanding of the relationship between ADC composition and biological disposition is necessary to ensure that the therapeutic window is not compromised by altered pharmacokinetics (PK), tissue distribution, and/or potential organ toxicity. The six-transmembrane epithelial antigen of prostate 1 (STEAP1) is being pursued as a tumor antigen target. To assess the role of ADC composition in PK, we evaluated plasma and tissue PK profiles in rats, following a single dose, of a humanized anti-STEAP1 IgG1 antibody, a thio-anti-STEAP1 (ThioMab) variant, and two corresponding thioether-linked monomethylauristatin E (MMAE) drug conjugates modified through interchain disulfide cysteine residues (ADC) and engineered cysteines (TDC), respectively. Plasma PK of total antibody measured by enzyme-linked immunosorbent assay (ELISA) revealed 45% faster clearance for the ADC relative to the parent antibody, but no apparent difference in clearance between the TDC and unconjugated parent ThioMab. Total antibody clearances of the two unconjugated antibodies were similar, suggesting minimal effects on PK from cysteine mutation. An ELISA specific for MMAE-conjugated antibody indicated that the ADC cleared more rapidly than the TDC, but total antibody ELISA showed comparable clearance for the two drug conjugates. Furthermore, consistent with relative drug load, the ADC had a greater magnitude of drug deconjugation than the TDC in terms of free plasma MMAE levels. Antibody conjugation had a noticeable, albeit minor, impact on tissue distribution with a general trend toward increased hepatic uptake and reduced levels in other highly vascularized organs. Liver uptakes of ADC and TDC at 5 days postinjection were 2-fold and 1.3-fold higher, respectively, relative to the unmodified antibodies. Taken together, these results indicate that the degree of overall structural modification in anti-STEAP1-MMAE conjugates has a corresponding level of impact on both PK and tissue distribution.