Highly Sensitive Fluorescence Response to Inclusion Complex Formation of Berberine Alkaloid with Cucurbit[7]uril
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- 作者：Mó ; nika Megyesi ; Lá ; szló ; Biczó ; k ; Istvá ; n Jablonkai
- 刊名：Journal of Physical Chemistry C
- 出版年：2008
- 出版时间：March 6, 2008
- 年：2008
- 卷：112
- 期：9
- 页码：3410 - 3416
- 全文大小：275K
- 年卷期：v.112,no.9(March 6, 2008)
- ISSN：1932-7455
文摘
The effect of inclusion complex formation on the fluorescence properties of berberine, a clinically importantnatural alkaloid, was studied using cucurbit[7]uril as macrocyclic host compound. The formation of a verystable 1:1 inclusion complex led to about 500-fold fluorescence intensity enhancement, which facilitated thedetection of berberine even below nanomolar concentration. Addition of NaCl caused a significant change inthe association constant and the fluorescence characteristics of the complex, whereas the variation of theanion had a small effect. 1-Alkyl-3-methylimidazolium type ionic liquids altered the fluorescent propertiesof the berberine-cucurbit[7]uril complex much more efficiently than did NaCl. Time-resolved fluorescencestudies showed ternary complex formation. Because berberine fluorescence is insensitive to pH and increasessubstantially upon inclusion in cucurbit[7]uril, the reversible self-assembly of this host-guest pair may findanalytical application in enzyme assays.