Development of an in vitro quantal assay in primary cell cultures for a non-occluded baculo-like virus of penaeid shrimp

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• 作者：Tapay ; Lourdes M. ; Lu ; Yuanan ; Gose ; Remedios B. ; Nadala Jr ; Elpidio Cesar B. ; Brock ; J.A. ; et. al.
• 关键词：Quantal assay ; Penaeid shrimp ; Shrimp baculo-like virus
• 刊名：Journal of Virological Methods
• 出版年：1997
• 出版时间：February, 1997
• 年：1997
• 卷：64
• 期：1
• 页码：37-41
• 全文大小：382 K

文摘

An in vitro quantal assay (TCID₅₀) for a non-occluded baculo-like virus isolate from naturally infected Penaeus japonicus obtained from China and experimentally infected P. stylirostris was developed using primary shrimp lymphoid cell cultures in Primaria^TM 24-well tissue culture plates. The virus caused cytopathogenic effect (CPE) in the cell cultures as early as 2 day post-infection (p.i.). Initially, the cells rounded up and finally detached from the culture vessel as the infection progressed. At the present time, there is no established quantitative in vitro cell culture protocol for the assay of this baculo-like virus which has been reported by our laboratory to be highly pathogenic for P. stylirostris and P. vannamei, the two species of penaeid shrimp commercially cultured in Hawaii and the Western hemisphere. This quantal assay thus provides a simple and convenient method for the detection and assay of infectious virus in cultured penaeid shrimp.