Electrochemical impedance spectroscopy detection of lysozyme based on electrodeposited gold nanoparticles

详细信息    查看全文

• 作者：Zhengbo Chen^a ; Lidong Li ; ^a ; ^{lilidong@buaa.edu.cn} ; ^{xhlili@163.com} ; Hongtao Zhao^a ; Lin Guo ; ^a ; ^{guolin@buaa.edu.cn} ; Xiaojiao Mu^a
• 关键词：Aptamer ; Electrochemical impedance spectroscopy ; Gold nanoparticles ; Lysozyme
• 刊名：Talanta
• 出版年：2011
• 出版时间：15 February 2011
• 年：2011
• 卷：83
• 期：5
• 页码：1501-1506
• 全文大小：818 K

文摘

A simple, highly sensitive, and label-free electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-lysozyme-aptamer as a molecular recognition element, was developed for the detection of lysozyme. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were electrodeposited onto the surface of a gold electrode, as a platform for immobilization of the aptamer. To quantify the amount of lysozyme, changes in the interfacial electron transfer resistance (R_et) of the aptasensor were monitored using the redox couple of an [Fe(CN)₆]^3−/4− probe. The R_et increased with lysozyme concentration. The plot of R_et against the logarithm of lysozyme concentration is linear over the range from 0.1 pM to 500 pM with a detection limit of 0.01 pM. The aptasensor also showed good selectivity for lysozyme without being affected by the presence of other proteins.