Validation and application of a quantitative real-time PCR assay to detect common wheat adulteration of durum wheat for pasta production

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• 作者：Elisa Carloni^a ; ^{elisa.carloni1@uniurb.it} ; Giulia Amagliani^a ; Enrica Omiccioli^b ; Veronica Ceppetelli^b ; Michele Del Mastro^c ; Luca Rotundo^a ; Giorgio Brandi^a ; Mauro Magnani^a
• 关键词：Common wheat adulteration ; Real-time PCR ; Semolina ; Durum wheat ; Pasta
• 刊名：Food Chemistry
• 出版年：2017
• 出版时间：1 June 2017
• 年：2017
• 卷：224
• 期：Complete
• 页码：86-91
• 全文大小：349 K
• 卷排序：224

文摘

Gliadin gene is not an appropriate common wheat (T. aestivum) specific target. Grain quantitative kit guarantees target detection, specificity, and sensitivity. The validated method is an effective system to quantify soft wheat in durum wheat. Commercial wheats and flours were analyzed to detect wheat adulteration.