Methylation Pattern of the CD14 and TLR2 Genes in Human Dental Pulp

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• 作者：Fabiano Pereira Cardoso ; DDS ; MSc^&lowast ; ; Simone Angé ; lica de Faria Amormino ; DDS ; MSc^&dagger ; ; Walderez Ornelas Dutra ; PhD^&dagger ; ; Ant&ocirc ; nio Paulino Ribeiro Sobrinho ; DDS ; MSc ; PhD^&lowast ; ; Paula Rocha Moreira ; DDS ; MSc ; PhD^&dagger ; ; ^{paularocha@ufmg.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CD14 ; dental pulp ; DNA methylation ; TLR2
• 刊名：Journal of Endodontics
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：40
• 期：3
• 页码：384-386
• 全文大小：278 K

文摘

Pattern recognition receptors, such as toll-like receptor 2 (TLR-2) and TLR-4, participate in the activation of immune cells by microorganisms in dental pulp. However, the expression levels of pattern recognition receptors can be modulated by epigenetic factors, especially DNA methylation. In this study, the methylation status of the TLR-2 and CD14 (TLR4 co-receptor) genes in healthy and inflamed human dental pulp was examined.

Methods

The Methyl-Profiler DNA Methylation qPCR Assay was used to verify the DNA methylation patterns.

Results

No differences in the methylation patterns were observed between the 2 groups. Most DNA was unmethylated in both groups.

Conclusions

The hypomethylation of TLR2 and CD14 genes is a usual feature in human dental pulp.