Fluorescence bioanalytical method for urea determination based on water soluble ZnS quantum dots

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• 作者：Eka Safitri^a ; ^bAuthor Vitae ; Lee Yook Heng^a ; ^c ; ^{leeyookheng@yahoo.co.uk} ; ^{yhl1000@ukm.my}Author Vitae ; Musa Ahmad^d ; Tan Ling Ling^c
• 关键词：Quantum dots ; ZnS ; Urea biosensor ; Urease immobilization ; Fluorescent label
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：240
• 期：Complete
• 页码：763-769
• 全文大小：1335 K
• 卷排序：240

文摘

A new method for urea determination based on fluorescence from a urease bioconjugated ZnS QD. Bioconjugation of ZnS QDs to urease via amide bond to obtain the ZnS QDs-urease bioconjugate. Immobilized urease hydrolyses urea, pH changes caused the deprotonation of ZnS QDs and fluorescence intensity increases. The biosensor response shows wide dynamic range 4 × 10−9 M to 4 × 10−3 M and low detection limit (< 4 × 10−9 M). The method has been validated with a spectrophotometric method and advantage is the reduce use of enzyme for urea analysis.