Next-generation sequencing (NGS) in the identification of encephalitis-causing viruses: Unexpected detection of human herpesvirus 1 while searching for RNA pathogens
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- 作者：Karol Perlejewskia ; karol.perlejewski@wum.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Marta Popiela ; b ; mszabl@wp.pl" class="auth_mail" title="E-mail the corresponding author ; Tomasz Laskusa ; tlaskus@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Shota Nakamurac ; nshota@gen-info.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Daisuke Motookac ; daisukem@gen-info.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Tomasz Stokowyd ; tomasz.stokowy@k2.uib.no" class="auth_mail" title="E-mail the corresponding author ; Dariusz Lipowskie ; dariusz.lipowski@wum.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Agnieszka Pollakf ; a.pollak@ifps.org.pl" class="auth_mail" title="E-mail the corresponding author ; Urszula Lechowiczf ; u.lechowicz@ifps.org.pl" class="auth_mail" title="E-mail the corresponding author ; Kamila Caraballo Corté ; sa ; kcaraballo@wum.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Adam Stępieńg ; astepien@wim.mil.pl" class="auth_mail" title="E-mail the corresponding author ; Marek Radkowskia ; marek.radkowski@wum.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Iwona Bukowska-Ośkoa ; ibukowska@wum.edu.pl" class="auth_mail" title="E-mail the corresponding author
- 关键词：Encephalitis ; Next-generation sequencing ; Unknown etiology
- 刊名：Journal of Virological Methods
- 出版年：2015
- 出版时间：15 December 2015
- 年：2015
- 卷：226
- 期：Complete
- 页码：1-6
- 全文大小：834 K
文摘
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Ribo-SPIA and NGS were employed to identify HHV-1 in encephalitis.
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Metagenomics could be used to determine encephalitis causative agents.
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Extraction of total RNA enables identification of both DNA and RNA pathogens.