CD24 induces localization of β1 integrin to lipid raft domains

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• 作者：Steffen Runz ; Claudia T. Mierke ; Safwan Joumaa ; Jü ; rgen Behrens ; Ben Fabry ; Peter Altevogt
• 关键词：GPI-anchor ; CD24 ; Cell invasion ; Integrins ; Invasion ; Motility ; Membrane rafts ; Lipid rafts ; Transmigration
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2008
• 出版时间：4 January 2008
• 年：2008
• 卷：365
• 期：1
• 页码：35-41
• 全文大小：701 K

文摘

The expression of the glycosyl phosphatidylinositol (GPI)-anchored protein CD24 correlates with poor prognosis in a variety of carcinomas. However, little is known about the cellular mechanisms of the CD24-mediated effects. In this study, we present evidence that CD24 affects the lateral localization of β1 integrin. Using stably CD24-transfected A125 and MDA-MB-435S carcinoma cells we show that CD24 augments β1-dependent cell motility and stimulates transmigration and invasion across a monolayer of endothelial cells. Furthermore, as demonstrated by sucrose density gradient centrifugation and Western Blot analysis, CD24 recruits β1 integrin into lipid raft domains. We suggest that CD24 acts as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins.