Fluorescent real-time quantitative measurements of intracellular peroxynitrite generation and inhibition
详细信息 查看全文
- 作者：Zhen Luo ; zhenluo@aatbio.com ; Qin Zhao ; Jixiang Liu ; Jinfang Liao ; Ruogu Peng ; Yunting Xi ; Zhenjun Diwu
- 关键词：Peroxynitrite ; Fluorescence ; Microplate reader ; Flow cytometer ; Curcumin
- 刊名：Analytical Biochemistry
- 出版年：2017
- 出版时间：1 March 2017
- 年：2017
- 卷：520
- 期：Complete
- 页码：44-48
- 全文大小：574 K
- 卷排序：520
文摘
Peroxynitrite (ONOO-), a strong oxidant species, is produced by the reaction of nitric oxide (NO) and superoxide (O2.-) radicals. It plays an important role as a biological regulator in numbers of physiological and pathological processes. In this study, we developed fluorescence-based real-time quantitative measurements to detect intracellular ONOO-. The probe DAX-J2 PON Green showed high selectivity toward ONOO- over other competing species, and has been successfully applied in microplate reader and flow cytometer to quantitatively measure endogenous ONOO- production. Moreover, the results demonstrated the inhibitory effects of curcumin on intracellular ONOO- generation.