Application of flow cytometry to monitor assimilable organic carbon (AOC) and microbial community changes in water
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- 作者：Ahmed M. Elhadidy ; a2elhadi@uwaterloo.ca" class="auth_mail" title="E-mail the corresponding author ; Michele I. Van Dyke ; Sigrid Peldszus ; Peter M. Huck
- 关键词：Assimilable organic carbon ; Biopolymers ; Flow cytometer ; High nucleic acid bacteria ; Low nucleic acid bacteria
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：130
- 期：Complete
- 页码：154-163
- 全文大小：1382 K
文摘
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Adapted flow cytometry AOC method was shown to be reliable and reproducible.
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Indigenous bacteria from the same source were used as inoculum for the AOC test.
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Inoculum with acceptable yield was only achieved when grown using source water.
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Natural inoculum utilized both high (biopolymer) and low MW organic matter fractions.
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Reliable cell count and AOC data obtained from river water and drinking water plant