Secretory production of designed multipeptides displayed on a thermostable bacterial thioredoxin scaffold in Pichia pastoris
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- 作者：Gloria Spagnolia ; Angelo Bolchia ; Davide Cavazzinia ; Somayeh Pouyanfardb ; Martin Mü ; llerb ; martin.mueller@dkfz.de" class="auth_mail" title="E-mail the corresponding author ; Simone Ottonelloa ; s.ottonello@unipr.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pyrococcus furiosus thioredoxin ; Secretory production ; Pichia pastoris ; Recombinant peptide antigens ; Human papillomavirus minor capsid protein L2 ; Vaccine
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：150-157
- 全文大小：796 K
文摘
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One-step purification of secreted thioredoxin-displayed multipeptides expressed in Pichia pastoris.
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Application testing to a peptide epitope from human papillomavirus minor capsid protein L2.
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Immunogenicity of the Pichia-produced, endotoxin-free thioredoxin-L2 antigen.