High-level expression of l-glutamate oxidase in Pichia pastoris using multi-copy expression strains and high cell density cultivation
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- 作者：Wang YaPinga ; Rao Benb ; Yan Honga ; Han Ruia ; Li Lia ; Liao Ping'ana ; Ma Lixina ; malixing98@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：l-glutamate oxidase ; Pichia pastoris ; Exponential feeding ; Copy number ; Fermentation
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：108-114
- 全文大小：881 K
文摘
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l-glutamate oxidase (Streptomyces ghanaenis ATCC 14672) was expressed in Pichia pastoris for the first time.
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The DNA sequence of the gox gene was modified according to P. pastoris codon usage preferences.
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Multi-copy expression strategy could increase the yield of the recombinant l-glutamate oxidase.