Molecular cloning, expression and IgE-immunoreactivity of phospholipase A1, a major allergen from Polybia paulista (Hymenoptera: Vespidae) venom

详细信息    查看全文

• 作者：Amilcar Perez-Riverol^a ; ^{aperezriverol@gmail.com} ; Franco Dani Campos Pereira^a ; ^b ; ^{franko_mg@hotmail.com} ; Alexis Musacchio Lasa^c ; ^{alexis.musacchio@cigb.edu.cu} ; Luis Gustavo Romani Fernandes^d ; ^{luisgrf1982@gmail.com} ; José ; Roberto Aparecido dos Santos-Pinto^e ; ^{jrbio04@rc.unesp.br} ; Dé ; bora Lais Justo-Jacomini^a ; ^{dmjacomini@gmail.com} ; Gabriel Oliveira de Azevedo^f ; ^{gabriel.azevedo4@outlook.com} ; Murilo Luiz Bazon^a ; ^{bazonmurilo@gmail.com} ; Mario Sergio Palma^e ; ^{mspalma@rc.unesp.br} ; Ricardo de Lima Zollner^d ; ^{zollner@unicamp.br} ; Má ; rcia Regina Brochetto-Braga^a ; ^g ; ^{mrbbraga@rc.unesp.br}
• 关键词：Polybia paulista ; Venom ; Allergy ; Diagnosis ; Recombinant phospholipase A1 ; Immunoglobulin E (IgE)
• 刊名：Toxicon
• 出版年：2016
• 出版时间：15 December 2016
• 年：2016
• 卷：124
• 期：Complete
• 页码：44-52
• 全文大小：2564 K
• 卷排序：124

文摘

cDNA of Poly p 1 allergen from P. paulista venom glands was cloned and sequenced. High levels of rPoly p 1 were produced in E. coli and purified by IMAC. rPoly p 1 was recognized by sera from P. paulista venom-sensitized patients. Levels of sIgE reactivity of allergic patients' sera to nPoly p 1 and rPoly p 1 were similar.