A multispecies Lactobacillus- and Bifidobacterium-containing probiotic mixture attenuates body weight gain and insulin resistance after a short-term challenge with a high-fat diet in C57/BL6J mice

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• 作者：Sophie Holowacz^a ; ^{s.holowacz@larenasante.com" class="auth_mail" title="E-mail the corresponding author} ; Charlotte Guigné ; ^b ; Gé ; rald Chê ; ne^b ; Sandrine Mouysset^c ; Angè ; le Guilbot^a ; Christian Seyrig^a ; Marc Dubourdeau^b
• 关键词：CLAs ; conjugated linoleic acids ; CT ; control diet ; FIAF ; fasting-induced adipocyte factor ; GLP-1 ; glucagon-like peptide-1 ; HF ; high-fat diet ; HF-Pb ; high-fat diet with probiotic mixture ; 5-HPETE ; 5-hydroperoxyeicosatetraenoic acid ; IBS ; inflammatory bowel syndrome ; LPS ; lipopolysaccharide ; LTA4H ; leukotriene A4 hydrolase ; LTC4S ; leukotriene C4 synthase ; Pb ; probiotic mixture ; SREBP-1 ; sterol regulatory element-binding protein 1 ; T2D ; type 2 diabetes ; Treg cells ; T regulatory cells
• 刊名：PharmaNutrition
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：3
• 期：3
• 页码：101-107
• 全文大小：547 K

文摘

A multispecies Lactobacillus- and Bifidobacterium-containing probiotic mixture significantly reduced the adverse metabolic and inflammatory effects of a 14-week high-fat diet in wild-type C57/BL6J mice gavaged 5 days a week with the probiotic mixture or vehicle. Recent evidence indicates that the gut microbiome may play a decisive role in the onset of obesity and associated chronic metabolic diseases, such as type 2 diabetes, by modulating nutrient absorption and factors conducive to development of a persistent low-grade inflammatory state. By modifying the gut microbiome, probiotics might constitute an effective dietary strategy for managing these metabolic disorders. The tested probiotic mixture significantly attenuated the increase in body weight, serum glucose concentration and insulin resistance induced by the high-fat diet. Furthermore, it significantly reduced the up-regulation of expression of several genes encoding pro-inflammatory adipokines and leukotriene pathway enzymes (CCL-2, IL-6 and leukotriene C₄ synthase in adipose tissue, leukotriene C₄ synthase and leukotriene A₄ hydrolase in the gut). It also significantly counteracted the down-regulation of adipose tissue gene expression related to the anti-inflammatory adipokine adiponectin in mice fed the high-fat diet. These results suggest that the mechanism underlying the beneficial metabolic effects of the probiotic mixture might involve inhibition of gut and adipose tissue inflammation.