Methanotrophic production of polyhydroxybutyrate-co-hydroxyvalerate with high hydroxyvalerate content

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• 作者：Andrew J. Cal^a ; W. Dirk Sikkema^a ; Maria I. Ponce^a ; Diana Franqui-Villanueva^a ; Timothy J. Riiff^a ; William J. Orts^a ; Allison J. Pieja^b ; Charles C. Lee^a ; ^{Charles.Lee@ars.usda.gov" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Polyhydroxyalkanoate ; Copolymer ; Methanotroph
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：87
• 期：Complete
• 页码：302-307
• 全文大小：954 K

文摘

Type II methanotrophic bacteria are a promising production platform for PHA biopolymers. These bacteria are known to produce pure poly-3-hydroxybutyrate homopolymer (PHB). We isolated a strain, Methylocystis sp. WRRC1, that was capable of producing a wide range of polyhydroxybutyrate-co-hydroxyvalerate copolymers (PHB-co-HV) when co-fed methane and valerate or n-pentanol. The ratio of HB to HV monomer was directly related to the concentration of valeric acid in the PHA accumulation media. We observed increased incorporation of HV and total polymer under copper-free growth conditions. The PHB-co-HV copolymers produced had decreased melting temperatures and crystallinity compared with methanotroph-produced PHB.