Iteratively improving natamycin production in Streptomyces gilvosporeus by a large operon-reporter based strategy
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- 作者：Yemin Wang1 ; Zhengsheng Tao1 ; Hualiang Zheng ; Fei Zhang ; Qingshan Long ; Zixin Deng ; Meifeng Tao ; tao_meifeng@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：CFU ; colony-forming units ; FDA ; Food and Drug Administration ; NTG ; N-methyl-N&rsquo ; -nitro-N-nitrosoguanidine ; NRPS ; non-ribosomal peptide synthetase ; PKS ; polyketide synthase
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：38
- 期：Complete
- 页码：418-426
- 全文大小：934 K
文摘
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Improving industrial strains of Streptomyces through a novel selection system.
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Selection system utilizes neo reporter gene fused to natamycin biosynthetic operon.
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Mutagenesis increased natamycin production of an industrial strain by 3.4-fold.
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Expression of entire gene cluster increased in natamycin-overproducing mutants.