Development of blocking ELISA for detection of antibodies against H9N2 avian influenza viruses
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- 作者：Jianmei Yang ; Xiaoguang Dai ; Hongjun Chen ; Qiaoyang Teng ; Xuesong Li ; Guangyu Rong ; Liping Yan ; Qinfang Liu ; Zejun Li ; lizejun@shvri.ac" class="auth_mail" title="E-mail the corresponding author
- 关键词：Blocking ELISA ; Antibodies ; H9N2 subtype ; Avian influenza virus
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：229
- 期：Complete
- 页码：40-47
- 全文大小：1357 K
文摘
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A monoclonal antibody binding specifically to HA protein of H9N2 AIV was produced.
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A blocking enzyme-linked immunosorbent assay (bELISA) was established for detection of antibodies against H9N2 AIV.
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The bELISA offers potentially a high throughput, rapid, sensitive and specific method for H9N2 antibodies quantitation in various host species.