Selenium levels and Glutathione peroxidase activity in the plasma of patients with type II diabetes mellitus

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• 作者：Raquel Gonzá ; lez de Vega^a ; Marí ; a Luisa Ferná ; ndez-Sá ; nchez^a ; ^{marisafs@uniovi.es" class="auth_mail" title="E-mail the corresponding author} ; Juan Carlos Ferná ; ndez^b ; Francisco Vicente Á ; lvarez Mené ; ndez^b ; Alfredo Sanz-Medel^a ; ^{asm@uniovi.es" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Glutathione peroxidase ; Oxidative strees ; Speciation ; ICP-MS ; Enzymatic activity
• 刊名：Journal of Trace Elements in Medicine and Biology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：37
• 期：Complete
• 页码：44-49
• 全文大小：754 K

文摘

Selenium, an essential trace element, is involved in the complex system of defense against oxidative stress through selenium-dependent glutathione peroxidases (GPx) and other selenoproteins. Because of its antioxidant properties, selenium or its selenospecies at appropriate levels could hinder oxidative stress and so development of diabetes. In this vein, quantitative speciation of selenium in human plasma samples from healthy and diabetic patients (controlled and non-controlled) was carried out by affinity chromatography (AF) coupled on-line to inductively coupled plasma mass spectrometry (ICP-MS) and isotope dilution analysis (IDA).

Similarly, it is well known that patients with diabetes who exhibit poor control of blood glucose show a decreased total antioxidant activity. Thus, we evaluated the enzymatic activity of GPx in diabetic and healthy individuals, using the Paglia and Valentine enzymatic method, observing a significant difference (p < 0.05) between the three groups of assayed patients (healthy (n = 24): 0.61 ± 0.11 U/ml, controlled diabetic (n = 38): 0.40 ± 0.12 U/ml and non-controlled diabetic patients (n = 40): 0.32 ± 0.09 U/ml). Our results show that hyperglycemia induces oxidative stress in diabetic patients compared with healthy controls. What is more, glycation of GPx experiments demonstrated that it is the degree of glycation of the selenoenzyme (another species of the Se protein) what actually modulates its eventual activity against ROS in type II diabetes mellitus patients.