Comparison of conventional ribotyping and PCR-RFLP ribotyping for the analysis of endemic strains of Neisseria meningitidis isolated in a local community over 7 years

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• 作者：Verdú ; M. Esther ; Coll ; Pere ; Fontanals ; Dionisia ; March ; Francesca ; Pons ; Immaculada ; et. al.
• 关键词：Neisseria meningitidis ; Molecular typing ; Ribotyping ; rRNA internally transcribed spacer region amplification
• 刊名：FEMS Microbiology Letters
• 出版年：1999
• 出版时间：October 15, 1999
• 年：1999
• 卷：179
• 期：2
• 页码：247-253
• 全文大小：264 K

文摘

Conventional ribotyping was compared with the PCR amplification of the intergenic spacer region between 16S and 23S rRNA genes (PCR-RFLP ribotyping) when applied to the subtyping of sporadic Neisseria meningitidis strains. Thirty isolates out of a total of 36 meningococcal disease cases, reported as having occurred in a particular community over a 7-year endemic period, were analyzed by each of the methods. Only ribotyping with three restriction enzymes (EcoRI, ClaI and XhoI) gave acceptable discriminatory power for short-term epidemiological purposes. We conclude that conventional ribotyping is a suitable method for typing sporadic meningococcal strains and that it cannot be replaced by the more straightforward PCR-RFLP ribotyping method.