Effect of La³⁺ on heat production by mitochondria isolated from hybrid rice

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• 作者：Jie Dai ; Chuan-Ling Li ; Ye-Zhong Zhang ; Qi Xiao ; Ke-Lin Lei ; Yi Liu
• 关键词：Mitochondria ; Metabolism ; La³⁺ ; Mechanism ; Permeability transition
• 刊名：Thermochimica Acta
• 出版年：2008
• 出版时间：10 April 2008
• 年：2008
• 卷：470
• 期：1-2
• 页码：77-82
• 全文大小：587 K

文摘

The effect of lanthanum on mitochondria isolated from hybrid rice Fengyou 559 (Oryza sativa L.) was investigated. Through in vivo culture, low-dose La³⁺ promoted, but higher dose La³⁺, restrained mitochondrial heat production. However, through in vitro incubation, La³⁺ manifested only inhibitory action on mitochondrial energy turnover, the concentration required for 50 % and 100 % inhibition being 50.9 and 230.2 μM (57.6 nmol/mg protein), respectively. In addition, La³⁺, like Ca²⁺, induced rice mitochondrial swelling and decreased membrane potential (Δψ), which was inhibited by the specific permeability transition inhibitor cyclosporine A (CsA). The induction approached a constant limitation while mitochondrial metabolism was completely prevented by La³⁺, and microscopy observation showed a high disruption of inner mitochondrial membrane in this state. These results demonstrated that lanthanum influenced rice mitochondria in vivo and in vitro via different action pathways, and the latter involved the opening of rice mitochondrial permeability.