Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse
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- 作者：Masakazu Hashimotoa ; 1 ; mhashimo@fbs.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Yukiko Yamashitab ; Tatsuya Takemotob ; 1 ; takemoto.tatsuya@tokushima-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Electroporation ; CRISPR/Cas9 system ; Cas9 protein ; Genome editing
- 刊名：Developmental Biology
- 出版年：2016
- 出版时间：1 October 2016
- 年：2016
- 卷：418
- 期：1
- 页码：1-9
- 全文大小：1985 K
文摘
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An electroporation method to generate genetically modified mouse is established.
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Electroporation of Cas9/sgRNA into early pronuclear zygotes generates non-mosaic mutant embryos.
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The electroporation method does not require special skill and can treat hundreds of zygotes in a short time.