Characterization of Dehydrodolichyl diphosphate synthase gene in rainbow trout (Oncorhynchus mykiss

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• 作者：Alex ; er Rebl ; Eckhard Anders ; Klaus Wimmers ; Tom Goldammer
• 关键词：Dolichol ; Glycosylation ; Prenyltransferase ; Rainbow trout ; Selection strain
• 刊名：Comparative Biochemistry and Physiology, Part B
• 出版年：2009
• 出版时间：March 2009
• 年：2009
• 卷：152
• 期：3
• 页码：260-265
• 全文大小：914 K

文摘

Dehydrodolichyl diphosphate synthase (DHDDS) catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of Dolichol, a glycosyl carrier-lipid required for the co-translational modification of various proteins. The resulting glycoproteins play a role in several physiological and pathological processes. This manuscript characterizes the DHDDS-like gene from rainbow trout (Oncorhynchus mykiss) and its tissue-specific mRNA abundance in two different strains. The ubiquitous expression of DHDDS in trout indicates the essential function of the product Dolichol for metabolic processes. The comparison of the deduced amino acid sequence with DHDDS proteins from different vertebrate, invertebrate, and herbal species reveals a high degree of conserved amino acids and protein regions suggesting a common functional relevance. This is the first report of a prenyltransferase homologue from a teleostean species.