Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type

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• 作者：K.S. Im^&lowast ; ; ^&dagger ; ; ^&Dagger ; ; ^§ ; ; J.H. Kim^&lowast ; ; ^&dagger ; ; ^&Dagger ; ; A.J. Graef^&lowast ; ; ^&dagger ; ; ^&Dagger ; ; I. Cornax^&lowast ; ; ^&Dagger ; ; ^§ ; ; D.M. Seelig^&lowast ; ; ^&dagger ; ; ^&Dagger ; ; M.G. O'Sullivan^&lowast ; ; ^&Dagger ; ; ^§ ; ; R.C. Kovi^&lowast ; ; ^&Dagger ; ; ^§ ; ; J.F. Modiano^&lowast ; ; ^&dagger ; ; ^&Dagger ; ; ^{modiano@umn.edu}
• 关键词：dog ; enteropathy-associated T-cell lymphoma ; immunodeficient mouse model ; patient-derived tumour xenograft
• 刊名：Journal of Comparative Pathology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：156
• 期：1
• 页码：37-41
• 全文大小：1611 K
• 卷排序：156

文摘

The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.