Assessing the biofuel production potential of Botryococcus braunii strains by sensitive rapid qualitative chemotyping using chemometrically-assisted gas chromatography-mass spectrometry
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- 作者：Benjamin Moutela ; Marion André ; b ; Delphine Kucmaa ; Jack Legranda ; Dominique Grizeaua ; Jé ; ré ; my Pruvosta ; Olivier Gonç ; alvesa ; olivier.goncalves@univ-nantes.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：EI ; electron impact ; FA ; fatty acid ; (F)AME ; (fatty) acid methyl ester ; FC ; fold change ; FS ; full scan mode ; GC-FID ; gas chromatography coupled to a flame ionization detector ; GC&ndash ; MS ; gas chromatography coupled to mass spectrometry ; HCs ; hydrocarbons ; HC ; hierarchical clustering ; HCPC ; hierarchical clustering on principal component analysis ; ND ; not detected ; PCA ; principal component analysis ; TIC ; total ion current ; TLs ; total lipids ; TL-HCs ; total lipid hydrocarbons ; TL-FAs ; total lipid f
- 刊名：Algal Research
- 出版年：2015
- 出版时间：September 2015
- 年：2015
- 卷：11
- 期：Complete
- 页码：33-42
- 全文大小：1187 K
文摘
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A chemotyping approach was developed to characterize Botryococcus braunii lipid extract.
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Alkenes and botryococcenes were characterized by chemometrically selecting fragment ions.
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Chemical motifs of alkenes and botryococcenes were used to program the SIM-GCMS method.
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This SIM-GCMS chemotyping method was sensitive, specific and biomass-saving.