6,6′-Dimycoloyl trehalose from a rapidly growing Mycobacterium: an alternative antigen for tuberculosis serodiagnosis

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• 作者：Lí ; n ; Luz M. ; Segura ; Erika ; Hermida-Escobedo ; Carlos ; Lemassu ; Anne ; C&eacute ; sar Salinas-Carmona ; Mario
• 关键词：Tuberculosis diagnosis ; Glycolipids ; Cord factor ; Immunodiagnosis
• 刊名：FEMS Immunology and Medical Microbiology
• 出版年：2003
• 出版时间：May 15, 2003
• 年：2003
• 卷：36
• 期：1-2
• 页码：47-54
• 全文大小：331 K

文摘

Mycobacterial O-acyltrehaloses have been described as highly specific and sensitive reagents for tuberculosis immunodiagnosis. An O-acyltrehalose-containing lipid fraction from the rapidly growing Mycobacterium fortuitum was found to include additional antigens, which presented high cross-reactivity with sera from tuberculosis-infected patients. Based on a combination of selective chemical degradations, thin-layer-chromatography analyses and ¹H-nuclear magnetic resonance spectroscopy, the antigenic by-product was identified as 6,6′-dimycoloyl trehalose, the so-called cord factor. The lipid was purified and tested in ELISA for pulmonary tuberculosis serodiagnosis. Sensitivity and specificity of the test were found to be 66.6–74.1 % and 95.2–99.0 % , respectively, showing a slightly higher efficiency as compared to the ELISA performed using 6,6′-dimycoloyl trehalose from Mycobacterium tuberculosis. No cross-reactivity was found with sera from Nocardia-infected individuals.