First report on Babesia vogeli infection in dogs in the Philippines

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• 作者：Adrian P. Ybañ ; ez^a ; ^b ; ¹ ; Rochelle Haidee D. Ybañ ; ez^a ; ¹ ; MaxFrancis G. Talle^b ; ^c ; Mingming Liu^d ; Paul Franck Adjou Moumouni^d ; Xuenan Xuan^d ; ^{gen@obihiro.ac.jp}
• 关键词：Babesia vogeli ; Dogs ; PCR ; Philippines
• 刊名：Parasitology International
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：66
• 期：1
• 页码：813-815
• 全文大小：194 K
• 卷排序：66

文摘

Babesia vogeli is a tick-borne protozoal pathogen that infects erythrocytes. In Southeast Asia, this pathogen has only been reported in Thailand. In this study, nine dogs presented at three different veterinary clinics in Cebu City, Philippines were found positive for B. vogeli. DNA was extracted from blood samples and tested using a PCR for genus Babesia and a PCR specific for B. vogeli (both based on the 18S rRNA gene). Blood smears (triplicate) from each sample were found negative. All positive amplicons were sequenced and were found to be 99.4% identical to registered B. vogeli sequences at Genbank. Phylogenetic analysis revealed monophyletic grouping of Philippine sequences with the registered A. platys Genbank sequences. This is the first report of B. vogeli infection in dogs in the Philippines.