Improvement of the stability and activity of immobilized trypsin on modified Fe3O4 magnetic nanoparticles for hydrolysis of bovine serum albumin and its application in the bovine milk
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- 作者：Keziban Atacana ; Bekir Ç ; akıroğlua ; Mahmut Ö ; zacara ; b ; mozacar@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ferric chloride hexahydrate (PubChem CID ; 24810) ; Ferrous sulfate heptahydrate (PubChem CID ; 62662) ; 4-Nitroaniline (PubChem CID ; 7475) ; Nα-Benzoyl-dl-arginine 4-nitroanilide hydrochloride ; BApNA (PubChem CID ; 2724371) ; Tris (hydroxymethyl) aminomethane (PubChem CID ; 6503) ; Tetramethylethylenediamine (PubChem CID ; 8037) ; β-Merkaptoetanol (PubChem CID ; 1567) ; Ammonium bicarbonate (PubChem CID ; 14013) ; Ammonium persulfate (PubChem CID ; 62648) ; Sodium dodecyl sulfate (PubChem CID ; 3423265)
- 刊名：Food Chemistry
- 出版年：2016
- 出版时间：1 December 2016
- 年：2016
- 卷：212
- 期：Complete
- 页码：460-468
- 全文大小：1621 K
文摘
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Fe3O4 NPs was synthesized by coprecipitation method and modified by gallic acid (MNPs-GA).
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Trypsin enzyme was immobilized onto MNPs-GA.
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Immobilization enhanced free trypsin activity.
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A possible mechanism of trypsin immobilization onto Fe3O4 NPs was proposed.
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Hydrolysis of BSA by immobilized trypsin was successfully performed.