BRET-monitoring of the dynamic changes of inositol lipid pools in living cells reveals a PKC-dependent PtdIns4P increase upon EGF and M3 receptor activation
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- 作者：Jó ; zsef T. Tó ; tha ; b ; Gergő Gulyá ; sa ; Dá ; niel J. Tó ; tha ; Andrá ; s Ballaa ; b ; Gerald R.V. Hammondc ; d ; Lá ; szló ; Hunyadya ; b ; Tamá ; s Ballac ; Pé ; ter Vá ; rnaia ; varnai.peter@med.semmelweis-univ.hu" class="auth_mail" title="E-mail the corresponding author
- 关键词：DAG ; diacylglycerol ; PPIns ; phosphoinositides ; RTK ; receptor tyrosine kinase ; GPCR ; G-protein coupled receptor ; Ins(1 ; 4 ; 5)P3 ; inositol 1 ; 4 ; 5-trisphosphate ; PM ; plasma membrane ; BRET ; bioluminescence resonance energy transfer ; PLC ; phospholipase C ; PtdIns(4 ; 5)P2 ; phosphatidylinositol 4 ; 5-bisphosphate ; PtdIns4P ; phosphatidylinositol 4-phosphate ; PI4K ; phosphatidylinositol 4-kinase ; EGFR ; epidermal growth factor receptor ; M3R ; type-3 muscarinic receptor ; PKC ; protein kinase C ; PtdIns(3 ; 4 ; 5)P3 ; phos
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：1861
- 期：3
- 页码：177-187
- 全文大小：1744 K
文摘
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Development of a BRET-based tool-set to monitor PPIn dynamics in live cells
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EGFR and moderate M3R activation lead to increased PtdIns4P level at the PM.
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RTK and GPCR stimulation can lead to PI4KA activation through a PKC-dependent manner.
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PM PtdIns4P synthesis is critical for Ins(1,4,5)P3 signaling upon receptor activation.