Over expression of miR-200c suppresses invasion and restores methotrexate sensitivity in lung cancer A549 cells
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- 作者：Wulin Shan1 ; Xiaolei Zhang1 ; Ming Li ; 18919685630@sina.cn" class="auth_mail" title="E-mail the corresponding author ; Fang Deng ; Jing Zhang
- 关键词：MTX ; methotrexate ; miRNAs ; microRNAs ; HDAC ; histone deacetylase ; A549 ; MTX-sensitive cell line A549 ; A549/MTX ; MTX-resistant cell line A549 ; RT-PCR ; real time-PCR ; MTT ; 3-(4 ; 5-dlmethylthiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; FCM ; flow cytometry
- 刊名：Gene
- 出版年：2016
- 出版时间：30 November 2016
- 年：2016
- 卷：593
- 期：2
- 页码：265-271
- 全文大小：1041 K
文摘
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MiR-200c was down-regulated in methotrexate-resistant cell line A549.
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MiR-200c acted as a tumor suppressor gene in lung cancer cells.
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Over expression of miR-200c reduced the resistance of A549 cells to methotrexate through the P53/P21 pathway.
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Over expression of miR-200c inhibited cell migration and invasion through the EZH2/E-cad pathway.